شناسایی مولکولی ژ نهای متالوبتالاکتامازی در پسودوموناس آئروژینوزا جداشده از زخم سوختگی

Background & Objectives: Pseudomonas aeruginosa is a leading cause of Hospital-acquired infection worldwide. The organisms isolated are often resistant to multiple antimicrobials particularly which are metallo-beta-lactamases (M&betaL) producers. Therefore, the present study was designed to molecular detection of Metallo-beta-lactamases genes in P. aeruginosa isolated from the burn patients. Materials and methods: This descriptive, cross-sectional study was performed between October to March 2014 on the 55 no duplicative and non-repetitive samples collected from burn patients in Tehran, Iran. Multiplex-PCR assay was done in order to detection of IMP ،VIM ،SPM ،GIM-1 and SIM-1 genes in the strains. Results: The average age of the population studied was 49 years, with a range of 15 to 89 years. Forty-three (78.2%) were male and 12(21.8%) were female. the distribution of the IMP ،VIM ،SPM ،GIM-1 and SIM-1 genes were 2(3.63%), 0(0.00%), 2(3.63%), 2(3.63%) and 0(0.00%), respectively. Conclusion: M&betaL producing P. aeruginosa is an emerging threat in burn patients and a cause of concern for physicians treating such infections.